C - Terminal AggregationIdentification of AD 74 , a Proteolytic Product of Enterococeus . tiiecalts Substance : Application of Liquid ChromatographylMass Spectrometry

Sexual aggregation inyo}yed in coajugative transfer of Ehterococcus,faecalis plasmid pADI is enhanced by the sex pheremone cADI, which is excreted from recipient cells. A membrane-anchored 137 kDa protein is a pADI-encoded aggregation substance designated asal, which is responsible for cell-cell contact and leads to the aggregation of cells. An AD74 protein is a proteolytic product corresponding to the N-terminal half of asal. The C-terminal of AD74 was identified as lysine at position 510 (K-510) by liquid